Postdoctoral/PhD Position: Structural biology research opportunity focused on investigating the type IV pili machineries, essential bacterial nanomachines involved in the assembly and disassembly of filamentous protein structures. The project aims to study the mechanisms and functions of these molecular machines, which could be used as targets for drugs. This will be done using advanced techniques such as mass spectrometry-based cross-linking, single particle cryo-electron microscopy (Cryo-EM), and Cryo-Electron Tomography.The position is hosted at the Pathogenesis of Vascular Infections Unit, Institut Pasteur, Paris, France.
Designation: Postdoctoral/PhD Position
Research Area: Structural biology, biochemistry, or related disciplines
Location: Paris, France
Eligibility/Qualification:
- MSc or PhD in structural biology, biochemistry, or related disciplines.
- Experience in protein purification, sample characterization, and cryo-EM/cryo-ET data acquisition/processing.
- Basic programming skills recommended.
- Comfortable with interdisciplinary science, including biophysics, microbiology, immunology, and engineering.
- Excellent communication and presentation skills in English.
Job Description:
The chosen candidate will work with a team of experts from different fields (biochemistry, microbiology, cell biology, biophysics, and animal models of infection) in the Pathogenesis of Vascular Infections Unit.The project involves employing innovative biochemical approaches to investigate protein nanomachines associated with bacterial secretion systems. Responsibilities include protein purification, enzymatic assays, single particle Cryo-EM, and Cryo-Electron Tomography. The candidate will have access to state-of-the-art facilities, including TFS Aquilos, Glacios, and Titan Krios microscopes for Cryo-EM and Cryo-ET, available at Institut Pasteur.
How to Apply: Interested candidates should submit their CV and motivation letter to Guillaume Duménil at guillaume.dumenil@pasteur.fr. Please address your application to Institut Pasteur, Unité “Pathogenèse des infections vasculaires”, 28 Rue du Dr Roux, 75015 Paris, France. For inquiries, contact 01 44 38 93 83.
Last Date for Apply: Until position filled
Table 1: Key Research Techniques
| Technique | Description |
|---|---|
| Mass spectrometry-based in situ cross-linking | Analytical technique for identifying protein-protein interactions in situ. |
| Single particle cryo-electron microscopy (Cryo-EM) | Method for determining the three-dimensional structures of macromolecules. |
| Cryo-Electron Tomography | Imaging technique used to visualize the three-dimensional structure of biological samples at high resolution. |
Table 2: Facilities at Institut Pasteur
| Facility | Description |
|---|---|
| TFS Aquilos | Microscope for Cryo-EM and Cryo-ET imaging. |
| Glacios | Microscope for Cryo-EM imaging. |
| Titan Krios | High-resolution Cryo-EM microscope for detailed structural analysis of biological samples. |
